Hybridization of Euglena gracilis chloroplast and nuclear DNA.

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Complete sequence of Euglena gracilis chloroplast DNA.

We report the complete DNA sequence of the Euglena gracilis, Pringsheim strain Z chloroplast genome. This circular DNA is 143,170 bp, counting only one copy of a 54 bp tandem repeat sequence that is present in variable copy number within a single culture. The overall organization of the genome involves a tandem array of three complete and one partial ribosomal RNA operons, and a large single co...

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Chloroplast DNA of the protozoan flagellate, Euglena gracilis, exists as circular molecules, 40 mum in contour length, as shown by electron microscopy and buoyant density analyses.

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Nucleotide Sequence Homology Exists between the Chloroplast and Nuclear Ribosomal DNAs of Euglena gracilis.

The nuclear and chloroplast ribosomal DNAs from Euglena were shown to have specific regions of nucleotide sequence homology. The regions of homology were identified by hybridization of restriction endonuclease DNA fragments of cloned chloroplast and nuclear ribosomal DNAs to one another. The regions of homology between these two ribosomal DNAs were in that part of the genes that code for the 3'...

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The presence of extrachromosomal nuclear ribosomal DNA (rDNA) in the unicellular alga Euglena gracilis has been established. This rDNA is circular. Each circle is 3.8 micron long and contains one rDNA unit. Oligomers are rare. Extrachromosomal rDNA is present in large amounts during the exponential phase of growth and appears less abundant during the stationary phase. It was found in all wild-t...

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ژورنال

عنوان ژورنال: Proceedings of the National Academy of Sciences

سال: 1967

ISSN: 0027-8424,1091-6490

DOI: 10.1073/pnas.57.1.156